Focusing Issues

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  • #4633

    w334amw
    • New User

    Back again…

    I have no problem focusing my samples on the 10x and 40x, but once I move up to 60x and 100x there seems to be just blackness. I have tried using the fine focus in both directions and I have moved the iris as well. Any suggestions on what I am doing wrong?

    Much appreciated.

    #4634

    Stewart Mills
    Keymaster
    • New User

    Are you using immersion oil? The 60x and 100x are oil contact objectives, not air.

    #4635

    w334amw
    • New User

    Yes. I am using the oil provided by the company.

    #4636

    Stewart Mills
    Keymaster
    • New User

    The only other possibility is that the illuminator is not properly focused in the center field of view.

    It may be best that we skype so I can see your set-up and better assist you. Please email me at stewart.mills(at)cytoviva.com with your contact information so we can set-up a time maybe tomorrow or Tuesday.

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