alex

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  • #3911


    alex

    • Junior Member
    • ★★★

    Hello Jamie,

    Do you have any new information about this subject?

    I am not yet contacted by the your office.

    I checked, and the pattern also appears in the darkfiled images, as you can see in the image I am attaching.

    Regards,
    Alexandre

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    #3907


    alex

    • Junior Member
    • ★★★

    Hello,

    Thanks for the answer!

    It started one or two months after the system installation.
    An additional information: the pattern is always in the same palace, even when we rotate the camera. Maybe this is not a problem with the CCD.

    Regards,
    Alex

    #3731


    alex

    • Junior Member
    • ★★★

    Hi

    I recorded the spectra of Au NPs, using the enhanced dark field in transmission mode.

    The spectra is similar to the one in the article I am atacching.

    Can/should I perform some type of correction in order to take in account the lamp profile?

    Thanks in advance!
    Alex

    #3608


    alex

    • Junior Member
    • ★★★

    Hi

    After trying to observe Au NPs, on top of a glass slide, using the enhanced dark field in transmission mode, I was able to record a spectra with that probably correspond to the scattering of the Au NPs. But I also measured other scattering that probably corresponds to dust or defects of the slide that also scattered the light. Is there some tool of the Cytoviva software that can perform a correction that would “subtract” this two scattering spectra?

    Thanks in advance!
    Alex

    #3600


    alex

    • Junior Member
    • ★★★

    Thanks for the help!

    The largest NPs in the population should have 11 nm of diameter. But maybe I can observe aggregates.

    I am trying to use the enhanced darkfield (in transmission mode).

    Does it requires a special type of filter placed in the fluorescent cube torrent?

    How can I check if the alignment between the condenser and the objective is correct?

    Thanks in advance!
    Alexandre

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