CytoViva-Jamie

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  • #4413


    CytoViva-Jamie

    Moderator

      Alex,
      Can you please e-mail me off the forum to solve this as it will be a more efficient way of communication?
      My e-mail is jamie.uertz@cytoviva.com

      Thank You!
      Jamie

      #4412


      CytoViva-Jamie

      Moderator

        Try it without the other software running.

        #4410


        CytoViva-Jamie

        Moderator

          Alex,
          No news yet. I am trying to get an answer before you re-install because that is a last resort!
          Have you tried to unplug the camera connected to the spectrograph and rest the power?
          Also is there any other software running while this happens??

          #4391


          CytoViva-Jamie

          Moderator

            Alex,
            Hello! This is Jamie Uertz with CytoViva. Before we re-install your software let me ask another co-worker about this error.
            Thank You!
            Jamie

            #4384


            CytoViva-Jamie

            Moderator

              Alexandre,
              Hello! So the fluid is drying out and leaving salt crystals?
              Even with a cover slip the fluid should not evaporate so quickly… what is the cell culture medium you are using?
              You can put the cells on a slide with culture medium and then put a coverslip. This will prevent the medium from drying out.

              Thank You,
              Jamie

              #4360


              CytoViva-Jamie

              Moderator

                Yes, check those settings and also see of the spectrograph mounted on top of the microscope has not move to the left or right and is a little loose.
                I have attached an image that shows the black arrow of where the attachment is.
                If where it is attached is slightly tilted the image can be tilted. The pixels are still square so I wouldnt worry about the alignment between the camera and spectrograph.

                #4358


                CytoViva-Jamie

                Moderator

                  Hello! This is Jamie Uertz with CytoViva. Where are you located?
                  Is this an enhanced darkfield image or a reflected light image?
                  Did you make sure the objective magnification matched the setting in the Hyperspectral acquisition?

                  Thank You!
                  Jamie

                  #4047


                  CytoViva-Jamie

                  Moderator

                    Andreas,
                    Because the spectral resolution is slightly different for each system it does not seem possible to combine the VNIR-SWIR spectra in ENVI.
                    But this may be possible to do in Excel. You can export any spectra in ENVI as ASCII data.
                    In Excel you can combine the 2 spectra and plot them.
                    Let me know if this works as I have never tried this!

                    #4043


                    CytoViva-Jamie

                    Moderator

                      Andreas,
                      You may be able to take a spectra from the VNIR image and a spectra from the SWIR image and overlay them in the same plot but I am not sure.
                      I will ask!
                      Thank You!
                      Jamie

                      #4041


                      CytoViva-Jamie

                      Moderator

                        Andreas,
                        Unfortunately there is no way to sync up the two images because the cameras for each have different imaging sizes. The SWIR camera has a smaller field of view.
                        But do you mean the joining just the spectrum in the graph?

                        Thank You,
                        Jamie

                        #3908


                        CytoViva-Jamie

                        Moderator

                          Alex,
                          Does it only really show up when the image background is bright?
                          There may be an IR filter you need to resolve this pattern. Elyse from our office will e-mail you with details.
                          Thanks!
                          Jamie

                          #3906


                          CytoViva-Jamie

                          Moderator

                            Alex,
                            This is indeed strange! Did this just start? Is the pattern always in the same place on the chip/image?
                            I will send this to Qimaging and see what they think.
                            Thank You!
                            Jamie

                            #3723


                            CytoViva-Jamie

                            Moderator

                              Jill,
                              The system itself does not have a PSF so to speak. The depth of focus depends on your magnification. Are you wanting to know the total distance a particular objective can focus?
                              Please e-mail me @ jamie.uertz@cytoviva.com with the details of what you you are trying to do with the 3D system.
                              Thanks!
                              Jamie

                              #3640


                              CytoViva-Jamie

                              Moderator

                                Jill,
                                Hello,this is Jamie Uertz with CytoViva. Are you wondering what the PSF is for your particular fluorophore? The camera itself has no PSF.
                                Thank You,
                                Jamie

                                #3609


                                CytoViva-Jamie

                                Moderator

                                  Alex,
                                  I would suggest the easiest fix for this issue is to use Ultra Clean glass slides. These can be acquired by the company Schott -Nexterion. We recommend Glass B. Here is the link:

                                  http://www.us.schott.com/nexterion/english/products/uncoated_slides/uncoated_slides.html?dimsdetected=1&ref=http%3A%2F%2Fwww.schott.com%2Fnexterion%2Fenglish%2Fproducts%2Funcoated_slides%2Funcoated_slides.html%3Fdimsdetected%3D1%26ref%3Dhttps%253A%252F%252Fwww.google.com%252F

                                  This will ensure that anything on your slides is your sample and not dust, scratches, etc.
                                  If you need ordering information we can help you with this!
                                  Let us know.
                                  Thank You!
                                  Jamie

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