CytoViva-Jamie

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  • #4360


    CytoViva-Jamie

    Moderator

      Yes, check those settings and also see of the spectrograph mounted on top of the microscope has not move to the left or right and is a little loose.
      I have attached an image that shows the black arrow of where the attachment is.
      If where it is attached is slightly tilted the image can be tilted. The pixels are still square so I wouldnt worry about the alignment between the camera and spectrograph.

      #4358


      CytoViva-Jamie

      Moderator

        Hello! This is Jamie Uertz with CytoViva. Where are you located?
        Is this an enhanced darkfield image or a reflected light image?
        Did you make sure the objective magnification matched the setting in the Hyperspectral acquisition?

        Thank You!
        Jamie

        #4047


        CytoViva-Jamie

        Moderator

          Andreas,
          Because the spectral resolution is slightly different for each system it does not seem possible to combine the VNIR-SWIR spectra in ENVI.
          But this may be possible to do in Excel. You can export any spectra in ENVI as ASCII data.
          In Excel you can combine the 2 spectra and plot them.
          Let me know if this works as I have never tried this!

          #4043


          CytoViva-Jamie

          Moderator

            Andreas,
            You may be able to take a spectra from the VNIR image and a spectra from the SWIR image and overlay them in the same plot but I am not sure.
            I will ask!
            Thank You!
            Jamie

            #4041


            CytoViva-Jamie

            Moderator

              Andreas,
              Unfortunately there is no way to sync up the two images because the cameras for each have different imaging sizes. The SWIR camera has a smaller field of view.
              But do you mean the joining just the spectrum in the graph?

              Thank You,
              Jamie

              #3908


              CytoViva-Jamie

              Moderator

                Alex,
                Does it only really show up when the image background is bright?
                There may be an IR filter you need to resolve this pattern. Elyse from our office will e-mail you with details.
                Thanks!
                Jamie

                #3906


                CytoViva-Jamie

                Moderator

                  Alex,
                  This is indeed strange! Did this just start? Is the pattern always in the same place on the chip/image?
                  I will send this to Qimaging and see what they think.
                  Thank You!
                  Jamie

                  #3723


                  CytoViva-Jamie

                  Moderator

                    Jill,
                    The system itself does not have a PSF so to speak. The depth of focus depends on your magnification. Are you wanting to know the total distance a particular objective can focus?
                    Please e-mail me @ jamie.uertz@cytoviva.com with the details of what you you are trying to do with the 3D system.
                    Thanks!
                    Jamie

                    #3640


                    CytoViva-Jamie

                    Moderator

                      Jill,
                      Hello,this is Jamie Uertz with CytoViva. Are you wondering what the PSF is for your particular fluorophore? The camera itself has no PSF.
                      Thank You,
                      Jamie

                      #3609


                      CytoViva-Jamie

                      Moderator

                        Alex,
                        I would suggest the easiest fix for this issue is to use Ultra Clean glass slides. These can be acquired by the company Schott -Nexterion. We recommend Glass B. Here is the link:

                        http://www.us.schott.com/nexterion/english/products/uncoated_slides/uncoated_slides.html?dimsdetected=1&ref=http%3A%2F%2Fwww.schott.com%2Fnexterion%2Fenglish%2Fproducts%2Funcoated_slides%2Funcoated_slides.html%3Fdimsdetected%3D1%26ref%3Dhttps%253A%252F%252Fwww.google.com%252F

                        This will ensure that anything on your slides is your sample and not dust, scratches, etc.
                        If you need ordering information we can help you with this!
                        Let us know.
                        Thank You!
                        Jamie

                        #3602


                        CytoViva-Jamie

                        Moderator

                          #3601


                          CytoViva-Jamie

                          Moderator

                            Alex,
                            Hello this is Jamie Uertz. How are you?
                            I would suggest checking out this video on our website to ensure your darkfiled alignment is correct.
                            After watching let us know if this helped and if you have any more questions!
                            Thanks!
                            Jamie

                            #3495


                            CytoViva-Jamie

                            Moderator

                              Alex,
                              Hello! Yes lines are normal as it is a line scanning system. Meaning a line of data ( 1 pixel row) is collected at a time. But these lines are pretty heavy. Meaning the signal to noise ratio is low on the image.
                              What are you imaging? Can you send an optical picture of what you are trying to scan?
                              This will help me.
                              Thanks!
                              Jamie

                              #3485


                              CytoViva-Jamie

                              Moderator

                                Hello Alex! No, unfortunately the spectral range is set inside the spectrograph so subsetting the range will have to be done after the scan is complete. This can be done in the ENVI software.
                                Thanks!
                                Jamie

                                #3280


                                CytoViva-Jamie

                                Moderator

                                  If you have followed all the instructions for importing a spectral library and ran the SAM routine and the result is a blank window then that means that no spectra were identified as a match.
                                  Unfortunately there is no video for mapping 2 separate libraries, just the document at the top of this topic thread.
                                  Thank You,
                                  Jamie

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