Enhanced Darkfield Microscopy

CytoViva Enhanced Darkfield Hyperspectral Imaging System

CytoViva® Enhanced Darkfield Microscope Optics

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics1.  This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop2.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system works by coupling the source illumination directly to the condenser optics. In this optical path, collimating lenses and mirrors align and fix the geometry of the light to match the geometry of the condenser annulus. This creates a very narrow, oblique angle of source illumination that can be precisely focused into the sample but bypasses the objective. The result is very intense  scatter from nanoscale samples against a very dark background.  Source illumination compatible with this system can be halogen, xenon or even laser, depending on the application.

Enhanced Darkfield Illumination Optics

CytoViva’s enhanced darkfield optics enable scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.


Finally, when combined with CytoViva’s Hyperspectral Imaging capability this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

Please email sales@CytoViva.com to request your private web demonstration.


1 Zhang, P., Park, S. and Kang, S. H. (2015), Microchip Electrophoresis with Enhanced Dark-Field Illumination Detection for Fast Separation of Native Single Super-Paramagnetic Nanoparticles. Bull. Korean Chem. Soc., 36: 1172–1177.

2 Chaudhari, K., & Pradeep, T. (2014). Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods. Scientific Reports, 4, 5948. http://doi.org/10.1038/srep05948