Darkfield Microscopy

CytoViva Darkfield Hyperspectral Imaging System

CytoViva® Enhanced Darkfield Microscopy

Unmatched Darkfield Microscopy for Nanoscale Optical Imaging

Observe Nanoparticles as Small as 10nm-20nm Right From Your Laboratory Benchtop

Darkfield microscopy has emerged as a critical tool for nanotechnology research. However, standard darkfield optics provided by the traditional microscope companies all operate similarly with inherent limitations in the way they manage source light to the condenser optics. This limitation significantly reduces their effectiveness and make them difficult to use.  As a result, hundreds of nanotechnology laboratories around the world have turned to the advanced performance of CytoViva’s patented enhanced darkfield microscope optics (US patents No. 7,542,203, 7,564,623).

CytoViva’s enhanced darkfield microscope optics are specifically designed to overcome the performance issues resident in standard darkfield optics. This improved performance has been accomplished by managing the source light onto the sample in a highly structured manner.  The result is an improvement in the scattering signal-to-noise ratio up to ten times when compared to traditional darkfield configurations. These results have been verified in multiple independent tests (see Figure 1 and 2). With CytoViva’s enhanced darkfield microscopy, nanomaterials as small as 10nm-20nm can be imaged right from your laboratory benchtop3.

Figure 1


Figure 2


CytoViva’s enhanced darkfield microscope optics work by coupling the source light directly to the darkfield condenser optics via light guide. These darkfield optics include, special collimating lenses and mirrors that align and fix the geometry of the light to match the geometry of the condenser. This creates a very narrow, oblique angle of light that can be precisely focused into the sample. The result is very intense scatter from nanoscale samples against a very dark background.  Source illumination compatible with this system can be halogen, mercury, xenon or even laser, depending on the application.

CytoViva’s enhanced darkfield microscopy enables scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials-based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast.

Finally, when combined with CytoViva’s Hyperspectral Imaging capability, this high signal-to-noise microscopy method enables researchers to spectrally characterize and map nanoscale samples in a wide range of environments.

CytoViva can provides full darkfield microscope systems and its enhanced darkfield optics can be added to most existing research grade optical microscopes.

Please email info@CytoViva.com to request your private web demonstration.

3Chaudhari, K., & Pradeep, T. (2014). Spatiotemporal mapping of three dimensional rotational dynamics of single ultrasmall gold nanorods. Scientific Reports, 4, 5948. http://doi.org/10.1038/srep05948